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The advanced basal plate regions of the caudal diencephalon (p2 and p1) and the midbrain (m1 and m2) are massively positive, having incorporated in the meantime the m2 portion (it is unclear whether m2 contributes to the oculomotor nucleus; note the distinct trochlear motor nucleus appears instead in the isthmic basal plate; III, IV; Fig. 8). The only exception showing less than massive neurogenesis in the midbrain basal plate is the presumptive rubral area (Ru; Fig. 8). As occurred at E12, the p3 basal plate shows instead a less populated pattern that resembles the neighboring portions of the hypothalamic basal domain.

The fact that some areas such as the tuber, the preoptic area and the alar prethalamus (excepting the prethalamic eminence) consistently show across diverse stages few or no AChE+ neurons supports the negative conclusions derived, that is, that AChE+ neurons are not present in the numbers expected. , 1993; Puelles et al. 2012a). There is therefore the possibility that such neurons hardly express AChE at postmitotic stages. 5 (Fig. 8), the missing basal cells may have been inadvertently scraped away while dissecting the specimens.

5A. A passes just under the cephalic flexure and is nearly transverse to p3 (presumptive reticular nucleus, Rt, identified in its alar part), though it continues rostrally into the alar hypothalamus, hp1 (hyp), at the level of the paraventricular population (arrow), and the telencephalon, hp1 (tel). Note also AChE+ cells in the mantle of the medial ganglionic eminence (MGE). B lies just above the cephalic flexure and crosses midbrain m1, basal p1 and p2, and alar p3, before reaching the telencephalon at the level of its stalk.

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